The pUC19 plasmid confers ampicillin resistance to its host through its bla gene which codes for a β-lactamase that cleaves the β-lactam ring in ampicillin. The pUC19 plasmid also contains the lacZ gene which encodes the N-terminal fragment of the enzyme beta-galactosidase.
What is pUC19 plasmid used for?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
What is the pUC19 plasmid?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. NEB offers a selection of common cloning plasmids and DNAs for use as substrates.
What is pUC19 DNA?
Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.
What does pUC19 mean?
plasmid cloning
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.
Is pUC19 a high copy plasmid?
Is pUC19 artificial?
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
Which selection system is used in the pUC19 plasmid?
triclosan selection
Vectors containing fabI enable triclosan selection. To test the potential of fabI as a selective marker for cloning, two vectors derived from pUC19 were constructed. First, we constructed pFab, where fabI together with its native promoter replaces the ampicillin resistance gene (bla) in pUC19.
How do you identify vectors?
(i) It should be small in size and of low molecular weight, less than 10 Kb (kilo base pair) in size so that entry/ transfer into host cell is easy. (ii) Vector must contain an origin of replication so that it can independently replicate within the host.
What is the function of the pUC19 gene?
In addition to β-galactosidase, pUC19 also encodes for an ampicillin resistance gene (ampR), via a β-lactamase enzyme that functions by degrading ampicillin and reducing its toxicity to the host. The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number.
Which media should be used for pUC19 plasmid cloning?
Therefore, the media used should contain ampicillin, IPTG, and X-gal . Due to its extensive use as a cloning vector in research and industry, pUC19 is frequently used in research as a model plasmid.
What is the origin of replication of pUC19?
The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number.
How many base pairs does pUC19 have?
It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the most widely used vector molecules as the recombinants, or the cells into which foreign DNA has been introduced, can be easily distinguished from the non-recombinants based on color differences of colonies on growth media.
